Molecular cloning and expression analysis of glutathione reductase gene in Chlamydomonas sp ICE-L from Antarctica

作者：Ding, Yu^*; Liu, Ying; Jian, Ji-Chang; Wu, Zao-He; Miao, Jin-Lai

来源：Marine Genomics, 2012, 5: 59-64.

DOI：10.1016/j.margen.2011.11.001

• Enzyme
• Ice algae
• RACE-PCR
• Real-time PCR
• Salinity

A cDNA (GenBank ID: GU395492) encoding cytosolic glutathione reductase (named ICE-LGR) in Antarctic microalgae Chlamydomonas sp. ICE-L was successfully cloned by RT-PCR and rapid amplification of cDNA ends technique (RACE). The expression patterns of ICE-LGR under different salinity stresses were determined by real-time PCR. ICE-LGR cDNA has 1913 bp nucleotides with an open reading frame (ORF) of 1458 bp, encoding 485 amino acid residues. The deduced amino acid sequence shows 79% homology with glutathione reductase (GR) of Chlamydomonas reinhardtii. Activity assessment and mRNA expression analysis results showed that activity and expression level of GR in ICE-L cells were up-regulated under either high or low salinity. Together, our results revealed that ICE-LGR might play an important role in Antarctic ice algae Chlamydomonas sp. ICE-L acclimatizing to polar high salinity environment as well as low salinity. These results provide us valuable information on further investigating the molecular mechanism of ICE-LGR.

• 出版日期2012-3
• 单位国家海洋局第一海洋研究所; 广东海洋大学