Alteration of Interferon-alpha/beta Receptors in Chronic Hepatitis B Patients

作者：Meng Fanli; Wang Jiefei; Ge Jian; Fan Xiaopeng; Wang Bing; Han Liyan; Kisseleva Tatiana; Paik YongHan; Brenner David A; Wang Kai^*

来源：Journal of Clinical Immunology, 2011, 31(3): 521-532.

DOI：10.1007/s10875-011-9518-6

• Interferon-alpha/beta receptor
• chronic hepatitis B
• interferon-alpha
• peripheral blood mononuclear cells (PBMCs)

Background The present study determined type I interferon (IFN) receptor (interferon-alpha/beta receptor (IFNAR)) and its predicable role in interferon-alpha 2b treatment in chronic hepatitis B (CHB) patients.
Methods Expression of IFN-alpha/beta R-1 and IFN-alpha/beta R-2 in peripheral blood mononuclear cells and in liver tissue was measured by flow cytometry, immunofluorescence, immunohistochemistry and RT-PCR.
Results IFN-alpha/beta R-1 and IFN-alpha/beta R-2 in monocytes and lymphocytes increased in CHB patients. Expression of IFNAR-1 and IFNAR-2 in liver had positive correlation with HBV-DNA in liver tissue. Expression of IFN-alpha/beta R in lymphocytes and monocytes increased in the first month, but then decreased during the subsequent interferon-alpha 2b treatment, patients who had higher levels of IFN-alpha/beta R-2 in monocytes prior to therapy showed better viral response than those with lower levels.
Conclusions Expression of IFN-alpha/beta R-2 in monocytes can be used as a predictable parameter to evaluate the effect of IFN-alpha treatment in CHB patients.

• 出版日期2011-6
• 单位山东大学; 复旦大学