delta-Amyrone, a specific inhibitor of cyclooxygenase-2, exhibits anti-inflammatory effects in vitro and in vivo of mice

作者：Niu Xiaofeng^*; Yao Huan; Li Weifeng; Mu Qingli; Li Huani; Hu Hua; Li Yongmei; Huang Huimin

来源：International Immunopharmacology, 2014, 21(1): 112-118.

DOI：10.1016/j.intimp.2014.04.019

• delta-Amyrone
• Inflammation
• Peritonitis
• Peritoneal macrophages
• Cyclooxygenase-2

The whole plant of Sedum lineare Thunb has been used as traditional folk medicines for the treatment of sore throat, persistent hepatitis, jaundice and dysentery. delta-Amyrone (13(18)-Oleanen-3-one), a pentacyclic triterpene compound from S. lineare Thunb, was found to possess a potent anti-inflammatory effect in different inflammation model animals. Pretreatment with delta-Amyrone (i.p.) inhibited the ear edema in xylene-induced mouse ear edema. delta-Amyrone also decreased the level of nitric oxide (NO), prostaglandin E-2 (PGE(2)), interleukin-6 (IL-6) and leukocyte numbers in acetic acid-induced peritonitis in vivo. To clarify the possible mechanism of delta-Amyrone, we investigated the effect of delta-Amyrone in lipopolysaccharide (LPS) induced peritoneal macrophages. The data indicated that delta-Amyrone notably inhibited IL-6, TNF-alpha and NO production. In addition, the result showed that delta-Amyrone may control the cyclooxygenase-2 (COX-2) regulation and not the cyclooxygenase-1 (COX-1) at protein levels. These results suggest that delta-Amyrone is a bioactive agent which possesses anti-inflammatory effects, which may be relevant to the regulation of COX-2.

• 出版日期2014-7
• 单位西安交通大学