Microbial acetyl-CoA metabolism and metabolic engineering

作者：Krivoruchko Anastasia; Zhang Yiming; Siewers Verena; Chen Yun; Nielsen Jens^*

来源：Metabolic Engineering, 2015, 28: 28-42.

DOI：10.1016/j.ymben.2014.11.009

• Yeast
• Bacteria
• Acetyl-CoA
• Central carbon metabolism
• Industrial biotechnology

Recent concerns over the sustainability of petrochemical-based processes for production of desired chemicals have fueled research into alternative modes of production. Metabolic engineering of microbial cell factories such as Saccharomyces cerevisiae and Escherichia coli offers a sustainable and flexible alternative for the production of various molecules. Acetyl-CoA is a key molecule in microbial central carbon metabolism and is involved in a variety of cellular processes. In addition, it functions as a precursor for many molecules of biotechnological relevance. Therefore, much interest exists in engineering the metabolism around the acetyl-CoA pools in cells in order to increase product titers. Here we provide an overview of the acetyl-CoA metabolism in eukaryotic and prokaryotic microbes (with a focus on S. cerevisiae and E. coli), with an emphasis on reactions involved in the production and consumption of acetyl-CoA. In addition, we review various strategies that have been used to increase acetyl-CoA production in these microbes.

• 出版日期2015-3