Depth-resolved measurement of mucosal microvascular blood content using low-coherence enhanced backscattering spectroscopy

作者：Radosevich Andrew J^*; Turzhitsky Vladimir M; Mutyal Nikhil N; Rogers Jeremy D; Stoyneva Valentina; Tiwari Ashish Kumar; De La Cruz Mart; Kunte Dhananjay P; Wali Ramesh K; Roy Hemant K; Backman Vadim

来源：Biomedical Optics Express, 2010, 1(4): 1196-1208.

DOI：10.1364/BOE.1.001196

Low-coherence enhanced backscattering (LEBS) spectroscopy is a light scattering technique which uses partial spatial coherence broadband illumination to interrogate the optical properties at sub-diffusion length scales. In this work, we present a post-processing technique which isolates the hemoglobin concentration at different depths within a sample using a single spectroscopic LEBS measurement with a fixed spatial coherence of illumination. We verify the method with scattering (spectralon reflectance standard and polystyrene microspheres) and absorbing (hemoglobin) phantoms. We then demonstrate the relevance of this method for quantifying hemoglobin content as a function of depth within biological tissue using the azoxymethane treated animal model of colorectal cancer.

• 出版日期2010-11-1
• 单位西北大学