Application of bioluminescence imaging to therapeutic intervention of herpes simplex virus type I-Thymidine kinase/ganciclovir in glioma

作者：Jang Su Jin; Kang Joo Hyun; Kim Kwang Il; Lee Tae Sup; Lee Yong Jin; Lee Kyo Chul; Woo Kwang Sun; Chung Wee Sup; Kwon Hee Chung; Ryu Chun Jeih; Choi Tae Hyun; Choi Chang Woon; Lim Sang Moo; Cheon Gi Jeong^*

来源：Cancer Letters, 2010, 297(1): 84-90.

DOI：10.1016/j.canlet.2010.04.028

• Glioma
• HSV1-tk
• Luciferase
• Molecular imaging
• Suicidal gene therapy

Lentiviral vector containing the HSV1-tk and firefly luciferase (fLuc) gene was infected into C6 and C6-TL expressing HSV1-tk and fLuc gene was generated. C6-TL showed higher [(125)I]IVDU uptake than C6. The survival rate of C6-TL decreased more rapidly with increasing GCV dose and was well correlated with fLuc activity. The images of microPET clearly demonstrated higher uptake of [(18)F]FHBG into the C6-TL tumor. Inhibition of tumor growth was observed in C6-TL tumor-bearing mice treated with GCV through tumor size measurement and bioluminescence imaging. The therapeutic effect of HSV1-tk/GCV system can be monitored using bioluminescent imaging and tumor size measurement.

• 出版日期2010-11-1