Application of Quantitative Real-Time Polymerase Chain Reaction on the Assessment of Organophosphorus Compound Degradation in in situ Soil

作者：Kwak Yunyoung; Kim Sang Jun; Rhee In Koo; Shin Jae Ho^*

来源：Journal of the Korean Society for Applied Biological Chemistry, 2012, 55(6): 757-763.

DOI：10.1007/s13765-012-2168-4

• biodegradation
• opd gene
• organophosphorus hydrolase
• quantitative real-time polymerase chain reaction

Quantitative real-time PCR (qPCR) method was applied to quantify the functional gene encoding organophosphorus hydrolase for assessing the degradation efficacy by bacterial strains on an organophosphorus compound in in situ soil. The specific primers targeting the organophosphorus hydrolase were designed and tested on reference bacterial strains and in DNA samples extracted from in situ soil samples contaminated by an organophosphorus compound. The established qPCR assay is a practical method for the analysis of in situ soil samples undergoing bioremediation of organophosphorus compounds.

• 出版日期2012-12
• 单位中国人民解放军海军大连舰艇学院