Artificial microRNAs (amiRNAs) engineering - On how microRNA-based silencing methods have affected current plant silencing research

作者：Sablok Gaurav^*; Perez Quintero Alvaro L; Hassan Mehedi; Tatarinova Tatiana V; Lopez Camilo

来源：Biochemical and Biophysical Research Communications, 2011, 406(3): 315-319.

DOI：10.1016/j.bbrc.2011.02.045

• amiRNAs
• Gene knockouts
• Model organism
• Virus

In recent years, endogenous microRNAs have been described as important regulators of gene expression in eukaryotes. Artificial microRNAs (amiRNAs) represent a recently developed miRNA-based strategy to silence endogenous genes. amiRNAs can be created by exchanging the miRNA/miRNA* sequence within a miRNA precursor with a sequence designed to match the target gene, this is possible as long as the secondary RNA structure of the precursor is kept intact. In this review, we summarize the basic methodologies to design amiRNAs and detail their applications in plants genetic functional studies as well as their potential for crops genetic improvement.

• 出版日期2011-3-18
• 单位华中农业大学