Highly regioselective and efficient production of 1-cyanocyclohexaneacetamide by Rhodococcus aetherivorans ZJB1208 nitrile hydratase

作者：Zheng, Ren-Chao; Yin, Xin-Jian; Zheng, Yu-Guo^*

来源：Journal of Chemical Technology and Biotechnology, 2016, 91(5): 1314-1319.

DOI：10.1002/jctb.4724

• Rhodococcus aetherivorans
• nitrile hydratase
• 1-cyanocyclohexaneacetamide
• regioselective
• high substrate loading

BACKGROUNDThe regioselective hydration of alicyclic ,-dinitrile 1-cyanocyclohexaneacetonitrile (1-CCHAN), followed by hydrogenation of -cyano group is a green and elegant route to gabapentin. As the selective chemical hydration of dinitrile is virtually impossible, a bioprocess for regioselective hydration of 1-CCHAN was developed using microbial nitrile hydratase. @@@ RESULTSA newly isolated NHase producing strain, Rhodococcus aetherivoransZJB1208, was successfully used for hydration of 1-CCHAN. Some key parameters of the biocatalytic process, including reaction temperature, pH, catalyst loading and substrate loading, were optimized. The fed-batch biotransformation was performed in non-buffered water system with the continuous precipitation of 1-cyanocyclohexaneacetamide. The substrate loading was increased up to 864 g L-1 (6.0 mol L-1), giving a product concentration of 966.7 g L-1 and biocatalyst yield (g(product)/g(cat)) of 204.2. @@@ CONCLUSIONThis study is the first example of microbial nitrile hydratase with both excellent regioselectivity and strong substrate tolerance for alicyclic dinitrile and affords a potentially industrial route to gabapentin.

• 出版日期2016-5
• 单位浙江工业大学