A new technique of in vivo synchrotron radiation coronary microangiography in the rat

作者：Sakamoto Hiroaki^*; Matsushita Shonosuke; Hyodo Kazuyuki; Tokunaga Chiho; Sato Fujio; Hiramatsu Yuji

来源：Acta Radiologica, 2015, 56(9): 1105-1107.

DOI：10.1177/0284185114549570

• Cardiac
• angiography
• animal investigations
• ischemia/infarction

Background: Previously, in our laboratory, synchrotron radiation coronary microangiography (SRCA) using Langendorff-perfused rat hearts could visualize a coronary artery of 50 mu m in diameter. However, in vivo rat SRCA poses the problem of compromised temporal resolution due to the rapid heart rate of rats.
Purpose: To establish a simple method of in vivo rat SRCA with bradycardia induced by intravenous injection of adenosine triphosphate disodium hydrate (ATP).
Material and Methods: SRCA was performed at the Photon Factory of the High Energy Accelerator Research Organization (Tsukuba, Japan). Eight male Wistar rats were anesthetized. A catheter for injecting the contrast material was inserted into the carotid artery. Temporary bradycardia was induced by an intravenous bolus injection of 5 mg of ATP, and SRCA was performed immediately thereafter.
Results: After ATP administration, the average heart rate decreased from 388 to 73 beats per minute. As a result, we could detect a coronary artery as small as 45 mu m in diameter.
Conclusion: Our SRCA system which has a high resolution of 9 mu m per pixel could detect a coronary artery as small as 45 mu m in diameter in the in vivo rat.

• 出版日期2015-9