Multiplex PCR and oligonucleotide microarray for detection of single-nucleotide polymorphisms associated with Plasmodium falciparum drug resistance

作者：Zhang Guo Qing; Guan Ya Yi; Sheng Hai Hui; Zheng Bin; Wu Song; Xiao Hua Sheng; Tang Lin Hua^*

来源：Journal of Clinical Microbiology, 2008, 46(7): 2167-2174.

DOI：10.1128/JCM.00081-08

Drug resistance in Plasmodium falciparum is a serious public health threat in the countries where this organism is endemic. Since resistance has been associated with specific single-nucleotide polymorphisms (SNPs) in parasite genes, molecular markers are becoming useful surrogates for monitoring the emergence and dispersion of drug resistance. In this study, a multiplex PCR (mPCR) and oligonucleotide microarray method was developed for the detection of these SNPs in genes encoding chloroquine resistance transporter (Pfcrt), multidrug resistance 1 (Pfmdr1), dihydrofolate reductase (Pfdhfr), dihydropteroate synthetase (Pfdhps), and ATPase 6 (PfATPase6) of P. falciparum. The results show that DNA microarray technology, combined with mPCR, is a promising and time-saving tool that supports conventional detection methods, allowing sensitive, accurate, simultaneous analysis of the SNPs associated with drug resistance in P. falciparum.

• 出版日期2008-7
• 单位中国疾病预防控制中心寄生虫病预防控制所; 中国疾病预防控制中心