A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

作者：Cui, Liang; Zhu, Zhi^*; Lin, Ninghang; Zhang, Huimin; Guan, Zhichao; Yang, Chaoyong James

来源：Chemical Communications, 2014, 50(13): 1576-1578.

DOI：10.1039/c3cc48707e

• ULTRASENSITIVE DETECTION
• MOLECULAR BEACONS
• EXPRESSION
• PROBES
• SYSTEM
• GENE

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA-CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

• 出版日期2014
• 单位厦门大学