Antidiabetic effects of chitooligosaccharides on pancreatic islet cells in streptozotocin-induced diabetic rats

作者：Liu Bing; Liu Wan Shun^*; Han Bao Qin; Sun Yu Ying

来源：World Journal of Gastroenterology, 2007, 13(5): 725-731.

• chitooligosaccharides
• diabetes mellitus
• two hours plasma glucose
• oral glucose
• tolerance test
• pancreastic islet cells
• streptozotocin

AIM: To investigate the effect of chitooligosaccharides on proliferation of pancreatic islet cells, release of insulin and 2 h plasma glucose in streptozotocin-induced diabetic rats.
METHODS: In vitro, the effect of chitooligosaccharides on proliferation of pancreatic islet cells and release of insulin was detected with optical microscopy, colorimetric assay, and radioimmunoassay respectively. In vivo, the general clinical symptoms, 2 h plasma glucose, urine glucose, oral glucose tolerance were examined after sixty days of feeding study to determine the effect of chitooligosaccharides in streptozotocin-induced diabetic rats.
RESULTS: Chitooligosaccharides could effectively accelerate the proliferation of pancreatic islet cells. Chitooligosaccharides (100 mg/L) had direct and prominent effect on pancreastic beta cells and insulin release from islet cells. All concentrations of chitooligosaccharides could improve the general clinical symptoms of diabetic rats, decrease the 2 h plasma glucose and urine glucose, and normalize the disorders of glucose tolerance.
CONCLUSION: Chitooligosaccha rides possess various biological activities and can be used in the treatment of diabetes mellitus.

• 出版日期2007-2-7
• 单位中国海洋大学