Anti-inflammatory properties of extracts from Chimonanthus nitens Oliv. leaf

作者：Sun, Qi; Zhu, Jiajin^*; Cao, Feiwei; Chen, Fengjia

来源：PLos One, 2017, 12(7): e0181094.

DOI：10.1371/journal.pone.0181094

• PERFORMANCE LIQUID-CHROMATOGRAPHY
• BIOACTIVE COMPOUNDS
• PHENOLIC-COMPOUNDS
• COMPREHENSIVE CHARACTERIZATION
• ANTIOXIDANT ACTIVITY
• IMMUNE-RESPONSES
• ZEBRAFISH LARVAE
• INFLAMMATION
• MODEL
• IDENTIFICATION

Chimonanthus nitens Oliv. (CN) is a species in the family Calycanthaceae. Its leaf is widely used to make traditional herbal tea in southern China and has a wide range of therapeutic effects. The profile of the ethanol extracts from CN leaves was identified by UPLC-QTOFMS/ MS. Forty seven compounds were determined including organic acids, phenolic acids and derivatives, flavonoids, coumarins, fatty acids and other compounds. The effect of the CN extracts on the inflammatory damage in zebrafish and in RAW 264.7 cells was investigated. The extracts demonstrated a strong ability to inhibit the recruitment of neutrophils in LPS-stimulated zebrafish, but macrophage migration was not significantly affected. Proinflammatory cytokines (i.e., TNF-alpha, IL-6 and IL-1 beta) were also determined by q-PCR. The extracts strongly reduced mRNA expression of TNF-alpha, IL-6 but not IL-1 beta in zebrafish model, while significantly inhibited the production of the factors in the RAW 264.7 cells. Therefore, our results suggest that the ethanol extracts of CN leaves may serve as a source of nutraceutical compounds with anti-inflammatory properties.

• 出版日期2017-7-10
• 单位浙江大学