科研之友
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摘要
Objective: To identify whether alpha-Klotho is a kind of acute phase protein and alternation in the level of protein and mRNA under restraint stress. Method: 48 mice were divided into three groups of 16: 1h, 10h and 20h treat time group. Each group included restraint stress and control subgroup with same number animals (n = 8). ELISA was utilized in the assay of serum alpha-Klotho and corticosterone, RT-PCR was occupied in the detection of the expression of alpha-Klotho mRNA in renal tissue. Result: alpha-Klotho protein concentration of control group 1h, 10h and 20h was 757.71 +/- 333.93 pg/ml, 687.38 +/- 342.79 pg/ml and 912.90 +/- 337.8 pg/ml, respectively. While the concentration of restraint stress group 1h, 10h and 20h was 726.40 +/- 342.79 pg/ml, 1261.54 +/- 442.71 pg/ml, and 1696.18 +/- 404.11 pg/ml. There was no significant difference among 1h, 10h and 20h control groups (P > 0.05) as well as 1h treat time subgroup. Compared with respective control group, the difference of restraint stress group in 10h and 20h treat time group was significant (P < 0.05). The expression of alpha-Klotho mRNA was slightly downregulated even when the mice underwent a 1 hour restraint stress, though without significance (p > 0.05). Prominent fold change, 2.02 and 2.46, happened in 10h and 20h restraint group with significance (P < 0.05 and P < 0.01), respectively. Conclusion: alpha-Klotho is a kind of acute phase protein. The serum alpha-Klotho protein is promoted while the alpha-Klotho mRNA is downregulated under the constraint stress.
- 出版日期2014
- 单位西安交通大学
