Xenon induces transcription of ADNP in neonatal rat brain

作者：Cattano Davide^*; Valleggi Simona; Ma Daqing; Kastsiuchenka Olga; Abramo Antonio; Sun Pamela; Cavazzana Andrea O; Natale Gianfranco; Maze Mervyn; Giunta Francesco

来源：Neuroscience Letters, 2008, 440(3): 217-221.

DOI：10.1016/j.neulet.2008.05.086

• suppression subtractive hybridization
• gene transcription
• neuroprotection
• anesthesia induced preconditioning

Xenon and other inhalational agents induce cell and organ protection through different and only partially elucidated molecular mechanisms. Anesthesia induced or pharmacologic preconditioning is a recognized mechanism of cell protection. In this study we explored the gene transcription of activity-dependent neuroprotective protein (ADNP) in neonatal rat brain as consequence to xenon exposure, comparing the noble gas to nitrogen. Seven-day-old Sprague Dawley rats were exposed for 120 min to 75% xenon and 25% oxygen or control condition consisting of 75% nitrogen and 25% oxygen (Air). ADNP was found to be differentially expressed by SSH, validated by Relative Real-Time PCR (RT-PCR) and confirmed by western blot and immunohistochemistry. The differential expression of ADNP in the rat neonatal brain may account for the preconditioning and neuroprotective effects exerted by gas xenon.

• 出版日期2008-8-8