Notch Signaling Downstream of foxD5 Promotes Neural Ectodermal Transcription Factors That Inhibit Neural Differentiation

作者：Yan Bo; Neilson Karen M; Moody Sally A^*

来源：Developmental Dynamics, 2009, 238(6): 1358-1365.

DOI：10.1002/dvdy.21885

• Notch signaling
• foxD5
• transcription factors
• neural differentiation

We investigated the role of the Notch signaling pathway in regulating several transcription factors that stabilize a neural fate and expand the neural plate. Increased Notch signaling in a neural lineage via a constitutively activated form (NICD) up-regulated geminin and zic2 in a cell-autonomous manner, and expanded the neural plate domains of sox11, sox2, and sox3. Loss- and gain-of-function assays show that foxD5 acts upstream of notch1 gene expression. Decreasing Notch signaling with an anti-morphic form of a Notch ligand (X-Delta-1(STU)) showed that the foxD5-mediated expansion of the sox gene neural plate domains requires Notch signaling. However, geminin and zic2 appear to be dually regulated by foxD5 and Notch1 signaling. These studies demonstrate that: (1) Notch signaling acts downstream of foxD5 to promote the expression of a subset of neural ectodermal transcription factors; and (2) Notch signaling and the foxD5 transcriptional pathway together maintain the neural plate in an undifferentiated state. Developmental Dynamics 238:1358-1365, 2009.

• 出版日期2009-6