A case study in converting disposable process scouting devices into disposable bioreactors as a future bioprocessing tool

作者：Vallejos Jose R; Uplekar Shaunak; da Silva Joao F; Brorson Kurt A; Moreira Antonio R; Rao Govind^*

来源：Biotechnology and Bioengineering, 2012, 109(11): 2790-2797.

DOI：10.1002/bit.24541

• disposable bioreactors
• optical sensors
• spinner flasks
• mass transfer
• mixing
• seed train

In this study, we perform mass transfer characterization (kLa) on a novel mechanically driven/stirred Process Scouting Device, PSD, (SuperSpinner D 1000 (R), SSD) and demonstrate that this novel device can be viewed as disposable bioreactor. Using patch-based optical sensors, we were able to monitor critical cell culture environmental conditions such as dissolved oxygen (DO) and pH in SSD for comparison to a 1?L standard spinner (SS) flask. We also coupled these mass transfer studies with mixing time studies where we observed relative high mixing times (5.2?min) that are typically observed in production scale bioreactors. Decreasing the mixing time 3.5-fold resulted in 30% increase in kLa (from 2.3 to 3.0?h-1) and minimum DO level increased from 0% to 20% for our model hybridoma cell line. Finally, maximum viable cell density and protein titer stayed within +/- 20% of historical data, from our standard 5?L stirred bioreactor (Biostat (R)) operated under active DO control. Biotechnol. Bioeng. 2012; 109: 27902797.

• 出版日期2012-11