New insights in endogenous modulation of ligand-gated ion channels: Histamine is an inverse agonist at strychnine sensitive glycine receptors

作者：Kletke Olaf; Sergeeva Olga A; Lorenz Philipp; Oberland Sonja; Meier Jochen C; Hatt Hanns; Gisselmann Guenter^*

来源：European Journal of Pharmacology, 2013, 710(1-3): 59-66.

DOI：10.1016/j.ejphar.2013.04.002

• Glycine
• Histamine
• Modulation
• Ligand-gated ion channel
• Endogenous modulator
• Competitive antagonist

Histamine is involved in many physiological functions in the periphery and is an important neurotransmitter in the brain. It acts on metabotropic H-1-H-4 receptors mediating vasodilatation, bronchoconstriction and stimulation of gastric acid secretion. In the brain histamine is produced by neurons in the tuberomamillary nucleus (TMN), which controls arousal. Histamine is also a positive modulator of the inhibitory Cys-loop ligand-gated ion channel GABA(A). We investigated now its effect on the second member of inhibitory Cys-loop ligand-gated ion channels, the strychnine sensitive glycine receptor. We expressed different human and rat glycine receptor subunits in Xenopus laevis oocytes and characterized the effect of histamine using the two electrode voltage clamp technique. Furthermore we investigated native glycine receptors in hypothalamic neurons using the patch-clamp technique. Histamine inhibited alp glycine receptors with an IC50 of 5.2 +/- 0.3 mM. In presence of 10 mM histamine the glycine dose response curve was shifted, increasing the EC50 for glycine from 25.5 +/- 1.4 mu M to 42.4 +/- 2.3 mM. In addition, histamine blocked the spontaneous activity of RNA-edited 0,313 glycine receptors. Histamine inhibited glycine receptors expressed in hypothalamic TMN neurons with an IC50 of 4.6 +/- 0.3 mM. Our results give strong evidence that histamine is acting on the same binding site as glycine, being an inverse agonist that competitively antagonizes glycine receptors. Thus, we revealed histamine as an endogenous modulator of glycine receptors.

• 出版日期2013-6-15