A Synthesized Glucocorticoid-Induced Leucine Zipper Peptide Inhibits Retinal Muller Cell Gliosis

作者：Gu, Ruiping; Ding, Xinyi; Tang, Wenyi; Lei, Boya; Jiang, Chen; Xu, Gezhi^*

来源：Frontiers in Pharmacology, 2018, 9: 331.

DOI：10.3389/fphar.2018.00331

• synthetic glucocorticoid
• induced leucine zipper peptide
• Muller cell
• gliosis
• NF-kappa B p65
• LPS

Purpose: The anti-inflammatory activities of protein glucocorticoid-induced leucine zipper (GILZ) have been demonstrated in vivo and in vitro. Here, we examined the potential effect of a synthetic peptide derived from the leucine zipper motif and proline-rich region of GILZ on suppressing inflammatory responses in primary cultured rat Muller cells. @@@ Methods: Peptides were selected from amino acids 98-134 of the GILZ protein (GILZ-p). Solid-phase peptide synthesis was used to generate the cell-penetrating peptide TAT, which was bound to the amino terminus of GILZ-p. Primary cultured retinal Muller cells were stimulated with lipopolysaccharide (LPS) alone or in combination with different concentrations of GILZ-p, and the interaction of GILZ-p with nuclear factor (NF)-kappa B p65 in Muller cells was investigated by western blotting, immunoprecipitation, and immunofluorescence. The expression of the Muller cell gliosis marker glial fibrillary acidic protein (GFAP), functional protein aquaporin (AQP)-4, and the inflammatory cytokines interleukin (IL)-1 beta, tumor necrosis factor (TNF) alpha, intercellular adhesion molecule (ICAM)-1, and monocyte chemoattractant protein (MCP)-1 was measured by Western Blotting. The concentration of those cytokines in culture medium was measured by using Enzyme-Linked Immunosorbent Assay. @@@ Results: The synthesized GILZ-p, which was water-soluble, entered cells and bound with NF-kappa B p65, inhibiting p65 nuclear translocation. GILZ-p inhibited the LPS-induced expression of GFAP, IL-1 beta, TNF alpha, ICAM-1, and MCP-1 in Muller cells and prevented the LPS-induced downregulation of AQP4. @@@ Conclusions: These results indicate that GILZ-p interacted with NF-kappa B p65 and suppressed p65 nuclear translocation, thereby inhibiting inflammatory cytokine release and Muller cell gliosis.

• 出版日期2018-4-6
• 单位复旦大学