Differential expression of mRNAs for M31 and M32, murine homologues of Drosophila heterochromatin protein 1 (HP1), during murine embryogenesis

作者：Miyado K; Sato M; Kimura M^*

来源：Biochemistry and Molecular Biology International, 1998, 44(5): 1051-1058.

• heterochromatin
• M31
• M32
• mRNA
• mouse
• HP1

Mice have two structural homologues of the Drosophila HP1 gene, termed M31 and M32, which have two structurally conserved domains, the chrome (C) and chrome shadow (CS) domains. In Drosophila, HP1 has been isolated as one of the components of conserved chromatin structure (heterochromatin) and is thought to bind DNA indirectly by mediating formation of a complex of nuclear proteins. However, little is known about the function of M31 and M32 in mammals. In order to assess the function of the M31 and M32 proteins, Northern blot analysis was performed in mice. M32 mRNA (with approximately 1,800 nucleotides (ntds)) was expressed strongly throughout embryonic stages examined and in TT2 embryonic stem (ES) cells, while expression of M31 mRNA (with approximately 2,300 and 1,100 ntds) was strong in embryos at embryonic day 8.5 (E8.5) and in TT2 cells, but was decreased at E12.5 and thereafter. Tn adults, expression of M32 mRNA was strongest in brain and brain stem among tissues examined, and was relatively weak in organs including kidney, stomach, intestine and testis. The two M31 transcripts were weakly but uniformly expressed throughout the organs examined in adults. These findings suggest that M31 and M32 may play different roles in murine embryogenesis, although they have the C and CS domains and are members of the same gene family.

• 出版日期1998-4
• 单位河北医科大学