Determination of glucose in human stomach cancer cell extracts and single cells by capillary electrophoresis with a micro-biosensor

作者：Wang, Xiaolei^*; Ma, Yanfang; Zhao, Man; Zhou, Minfeng; Xiao, Yan; Sun, Zifei; Tong, Lili^*

来源：Journal of Chromatography A, 2016, 1469: 128-134.

DOI：10.1016/j.chroma.2016.09.054

• Glucose
• Capillary electrophoresis
• Electrochemical biosensor
• Single-cell analysis

Bioactive species in cells can provide information about signal transduction, cell function, and the effects of disease treatment. In this article, a novel micro-biosensor was fabricated to detect glucose in individual human stomach cancer cells (MGC80-3 cells) with capillary electrophoresis (CE). We fabricated the micro-biosensors by immobilizing a single-walled carbon nanotube-glucose oxidase (GOx)-glutaraldehyde (GA) bio-composite at the palladium nanoparticle (PdNPs) modified Pt electrode. The linear concentration of glucose ranged from 2.0 mu M to 1.0 mM, with a detection limit of 0.5 mu M. Using this method, the mean amount of glucose in MGC80-3 cell extracts and in single cells was 20.0 fmol and 20 +/- 6 fmol (n = 10), respectively. The micro-biosensor exhibited high sensitivity, stability, and a long operating life, which are likely due to the biocompatible environment provided by BSA and GA, and the adsorption and faster electron transfer of SWNTs and PdNPs to GOx.

• 出版日期2016-10-21
• 单位山东师范大学