科研之友
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摘要
A fluorophore dyad (L) containing rhodamine B and naphthalimide units linked through C=N was constructed as a ratiometric probe for Cu2+. The selective fluorescent response of the probe L to Cu2+ was triggered by a Cu2+ induced ring-opening process of the rhodamine B spirolactam, which was based on an intramolecular fluorescence resonance energy transfer (FRET) mechanism from naphthalimide to rhodamine B. The system exhibited high selectivity for Cu2+ as compared to other tested metal ions and anions, and signaled the binding event through occurrence of FRET mediated emission at 580 nm of the rhodamine chromophore with enhancement in the fluorescence intensity, concurrently, the featured emission of the naphthalimide donor at around 525 nm was nearly completely quenched. Furthermore, it has been used for ratiometric imaging of Cu2+ in living cells with satisfying results.
- 出版日期2014
- 单位海南医学院
