Phytotoxicity of multi-walled carbon nanotubes on red spinach (Amaranthus tricolor L) and the role of ascorbic acid as an antioxidant

作者：Begum Parvin; Fugetsu Bunshi^*

来源：Journal of Hazardous Materials, 2012, 243: 212-222.

DOI：10.1016/j.jhazmat.2012.10.025

• Multi-walled carbon nanotubes
• Red spinach (Amaranthus tricolor L)
• Reactive oxygen species
• Phytotoxicity
• Ascorbic acid

Carbon nanotubes (CNTs) are a novel nanomaterial with wide potential applications: however the adverse effects of CNTs following environmental exposure have recently received significant attention. Herein, we explore the systemic toxicity and potential influence of 0-1000 mg L-1 the multi-walled CNTs on red spinach. The multi-walled CNTs exposed plants exhibited growth inhibition and cell death after 15 days of hydroponic culture. The multi-walled CNTs had adverse effects on root and leaf morphology, as observed by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Raman spectroscopy detected the multi-walled CNTs in leaves. Biomarkers of nanoparticle toxicity, reactive oxygen species (ROS), and cell damage in the red spinach were greatly increased 15 days post-exposure to the multi-walled CNTs. These effects were reversed when the multi-walled CNTs were supplemented with ascorbic acid (AsA), suggesting a role of ROS in the multl-walled CNT-induced toxicity and that the primary mechanism of the multi-walled CNTs%26apos; toxicity is oxidative stress.

• 出版日期2012-12