Antibody Detection against Red Sea Bream Iridovirus (RSIV) in Yellowtail Seriola quinqueradiata Using ELISA

作者：Kwon Se Ryun; Nishizawa Toyohiko^*; Takami Ikuo; Yoshimizu Mamoru

来源：Fish Pathology, 2010, 45(2): 73-76.

DOI：10.3147/jsfp.45.73

• antibody detection
• ELISA
• red sea bream iridovirus
• yellowtail

FBS components in viral culture fluid interfere with the detection of specific antibody in immunized fish. In the present study, we developed a method to eliminate the interference of FBS using a model of red sea bream iridovirus (RSIV) vaccine. In an experiment using yellowtail antiserum against BSA, which is a major component of FBS in cell culture media, antibodies against BSA were completely absorbed via incubation of antiserum in 50% FBS at 25 degrees C for 1 h. In antibody detection, ELISA with the sera of yellowtail surviving RSIV-challenge, antibodies against RSIV were detected, but those against FBS disappeared via the pre-treatment of the sera. Therefore, antibodies against RSIV are effectively detected using ELISA with the pre-treatment of fish sera.

• 出版日期2010-6