Hemoglobin and Streptococcus suis cell wall act in synergy to potentiate the inflammatory response of monocyte-derived macrophages

作者：Tanabe Shin ichi; Gottschalk Marcelo; Grenier Daniel^*

来源：Innate Immunity, 2008, 14(6): 357-363.

DOI：10.1177/1753425908098388

• Streptococcus suis
• cell wall
• hemoglobin
• macrophage
• cytokine
• kinase

Streptococcus suis, a major swine pathogen world-wide. call trigger macrophages to secrete large amounts of pro-inflammatory cytokines. which increase the permeability of the blood-brain barrier. In this study, we hypothesized that hemoglobin may potentiate the inflammatory response of human macrophages stimulated With a S. Suis Cell-wall preparation. Monocyte-derived macrophages were stimulated with the S. suis cell-wall preparation in the presence Or absence Of human hemoglobin, and the secretion of interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-8 was analyzed by enzyme-linked immunosorbent assays. The cell-wall preparation induced dose-dependent IL-1 beta. TNF-alpha, IL-6, and IL-8 responses in macrophages. Hemoglobin potentiated the cell-wall induced inflammatory response. resulting ill a significantly higher secretion of all the cytokines. The S. suis cell-wall preparation in combination with hemoglobin activated macrophage intracellular kinases involved in inflammatory signaling pathways. In conclusion, hemoglobin, which may be released ill vivo by the action of S. suis suilysin oil red blood cells. contributes to raising the levels of pro-inflammatory mediators by acting in synergy with S. suis cell-wall components. This phenomenon may contribute to the development and the severity of meningitis.

• 出版日期2008-12