Size Discrepancy: Not a Formidable Problem in a Mouse Heterotopic Aortic Transplant

作者：Wu Yuan; Bin Yejian; Yan Guoliang; Xu Shuangyue; Cheng Panpan; Huang Huabing; Zheng Jingxian; Qi Zhongquan^*

来源：Experimental and Clinical Transplantation, 2014, 12(4): 367-370.

DOI：10.6002/ect.2013.0209

• Heterotopic aortic transplant
• Mouse model
• Sleeve technique
• Thrombosis
• Size discrepancy

Objectives: We sought to develop a better approach in establishing a heterotopic aortic transplant model.
Materials and Methods: We used "sleeve" operation and set up 2 heterotopic aortic transplant experimental groups (groups 1 and 2). In group 1, donors (BALB/c (H-2(d)) mice; weight, 25-30 g) had the same weight as the recipients (C57BL/6 (H-2(d)) mice; weight, 25-30 g); in group 2, donors had lower weights (BALB/c (H-2d) mice, weight, 15-20 g). Grafts were examined macroscopically and histologically 60 days after the transplant.
Results: The thrombosis incidence was 10% in group 1 (5 of 50) and 2% in group 2 (1 of 50) (P < .05). Intestinal obstructions led to a high mortality rate in both groups: 8% in group 1 and 10% in group 2 (P < .05).
Conclusions: The issue of size discrepancy between donor and recipient aortas is not a problem in a heterotopic aortic transplant model with the use of a sleeve technique, while the high incidence of intestinal obstructions must be considered.

• 出版日期2014-8
• 单位厦门大学