Alternative Splicing and the Steady-State Ratios of mRNA Isoforms Generated by It Are under Strong Stabilizing Selection in Caenorhabditis elegans

作者：Barberan Soler Sergio^*; Zahler Alan M

来源：Molecular Biology and Evolution, 2008, 25(11): 2431-2437.

DOI：10.1093/molbev/msn181

• alternative splicing
• evolution
• microarrays

Evolutionary studies indicate that a high proportion of alternative splicing (AS) events are species-specific; just 28% of minor-form alternatively spliced exons are conserved between mice and humans. We employed a splicing-sensitive microarray to study the evolution of allele-specific AS in nematodes. We compared splicing levels among five distinct Caenorhabditis elegans lines. Our results indicate that AS is less variable between natural isolates (NIs) from England, Hawaii, and Australia than when compared with mutation accumulation lines (6% vs. 21%, respectively, vary compared with N2). This suggests that strong stabilizing selection shapes the evolution of the ratios of isoforms generated by AS in C. elegans. When we analyzed some of the splicing changes between the NIs, we found examples of changes in both cis and trans that lead to alterations in gene-specific AS. This indicates that both these mechanisms for changing AS are employed along the path toward speciation in nematodes.

• 出版日期2008-11