科研之友
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摘要
AMP-activated protein kinase (AMPK) is a highly conserved and multi-functional protein kinase that plays important roles in both intracellular energy balance and cellular stress response. In the present study, molecular characterization, tissue distribution and gene expression levels of the AMPK alpha 1 and alpha 2 genes from turbot (Scophthalmus maximus) under salinity stress are described. The complete coding regions of the AMPK alpha 1 and alpha 2 genes were isolated from turbot through degenerate primers in combination with RACE using muscle cDNA. The complete coding regions of AMPK alpha 1 (1722 bp) and alpha 2 (1674 bp) encoded 573 and 557 amino acids peptides, respectively. Multiple alignments, structural analysis and phylogenetic tree construction indicated that S. maximus AMPK alpha 1 and alpha 2 shared a high amino acid identity with other species, especially fish. AMPK alpha 1 and alpha 2 genes could be detected in all tested tissues, indicating that they are constitutively expressed. Salinity challenges significantly altered the gene expression levels of AMPK alpha 1 and alpha 2 mRNA in a salinity- and time-dependent manners in S. maximus gill tissues, suggesting that AMPK alpha 1 and alpha 2 played important roles in mediating the salinity stress in S. maximus. The expression levels of AMPK alpha 1 and alpha 2 mRNA were a positive correlation with gill Na+, K+-ATPase activities. These findings will aid our understanding of the molecular mechanism of juvenile turbot in response to environmental salinity changes.
