A metabolomic perspective of griseofulvin-induced liver injury in mice

作者：Liu Ke; Yan Jiong; Sachar Madhav; Zhang Xinju; Guan Ming; Xie Wen; Ma Xiaochao^*

来源：Biochemical Pharmacology, 2015, 98(3): 493-501.

DOI：10.1016/j.bcp.2015.09.002

• Griseofulvin
• Drug toxicity
• Drug metabolism
• Metabolomics
• Erythropoietic protoporphyria

Griseofulvin (GSF) causes hepatic porphyria in mice, which mimics the liver injury associated with erythropoietic protoporphyria (EPP) in humans. The current study investigated the biochemical basis of GSF-induced liver injury in mice using a metabolimic approach. GSF treatment in mice resulted in significant accumulations of protoporphyrin IX (PPIX), N-methyl PPIX, bile acids, and glutathione (GSH) in the liver. Metabolomic analysis also revealed bioactivation pathways of GSF that contributed to the formation of GSF-PPIX, GSF-GSH and GSF-proline adducts. GSF-PPIX is the precursor of N-methyl PPIX. A six-fold increase of N-methyl PPIX was observed in the liver of mice after GSF treatment. N-methyl PPIX strongly inhibits ferrochelatase, the enzyme that converts PPIX to heme, and leads to PPIX accumulation. Excessive PPIX in the liver results in bile duct blockage and disturbs bile acid homeostasis. The accumulation of GSH in the liver was likely due to Nrf2-mediated upregulation of GSH synthesis. In summary, this study provides the biochemical basis of GSF-induced liver injury that can be used to understand the pathophysiology of EPP-associated liver injury in humans.

• 出版日期2015-12-1
• 单位复旦大学