Detection of HPV in oral rinse samples from OPSCC and non-OPSCC patients

作者：Dang Juliet^*; Feng Qinghua; Eaton Keith D; Jang Hona; Kiviat Nancy B

来源：BMC Oral Health, 2015, 15(1): 126.

DOI：10.1186/s12903-015-0111-x

Background: Due to the increasing rates of oropharyngeal cancer, oral HPV infection is a significant concern.
Methods for detecting oral HPVs is not standardized as there are different techniques available. We propose that use of oral rinse samples to detect for HPVs is a suitable technique within a clinic setting. Thus, our main objective is to study HPV detection in oral rinse samples. Methods: In our study, we used oral rinse sample collection coupled with real-time PCR to detect for HPVs types 16 and 18, and preferentially amplified FAP PCR samples to detect for a broad range of HPVs, in oropharyngeal squamous cell carcinoma (OPSCC), non-OPSCC, and healthy patients.
Results: Thirty three percent of 100 cancer patients were positive for any type of HPV; of those 23 were positive for HPV16. Only 1 of 110 healthy controls was positive (this subject was positive for HPV18).
Conclusion: Our results indicate that HPV detection in oral rinse samples may be useful as a screening tool to detect HPV-associated oral cancers.

• 出版日期2015-10-15