An investigation of the antibacterial ability and cytotoxicity of a novel cu-bearing 317L stainless steel

作者：Sun, Da; Xu, Dake; Yang, Chunguang; Shahzad, M. Babar; Sun, Ziqing; Xia, Jin; Zhao, Jinlong; Gu, Tingyue; Yang, Ke^*; Wang, Guixue^*

来源：Scientific Reports, 2016, 6(1): 29244.

DOI：10.1038/srep29244

• RESISTANT STAPHYLOCOCCUS-AUREUS
• PANTON-VALENTINE LEUKOCIDIN
• COPPER SURFACE TOXICITY
• LISTERIA-MONOCYTOGENES
• SILVER NANOPARTICLES
• BACTERIAL VIABILITY
• ESCHERICHIA-COLI
• VIBRIO-CHOLERAE
• GENE
• INFECTIONS

In order to solve the challenging problem of microbial infections caused by microorganisms on medical implants, it is imperative to develop novel antimicrobial biomaterials. This work demonstrated that 317L-Cu stainless steel (SS), created by adding copper through a solution and aging heat treatment process, exhibited good antibacterial properties against staphylococcus aureus, achieving 2 log reduction of planktonic cells after 5 days of incubation. In this study, the antibacterial test was performed using the plate count method, the fluorescence cell staining method and the quantitative polymerase chain reaction (qPCR) method. It is well known that a high concentration of copper ion can lead to cytotoxicity. This work explored the cytotoxicity of 317L-Cu SS through real-time cell analysis (RTCA). Experimental results demonstrated that the 317L-Cu SS possessed a satisfactory antibacterial ability against S. aureus, and the antibacterial rate based on the reduction of sessile cell count reached 98.3% after 24-hour treatment. The bacterial adhesion and the biofilm thickness were considerably reduced by the 317L-Cu SS. The results of RTCA suggested that 317L-Cu SS did not introduce cytotoxicity to mouse cells, indicating its suitability as a medical implant material.

• 出版日期2016-7-7
• 单位辽宁大学; 中国科学院; 重庆大学; 中国科学院金属研究所