Antitumor effects evaluation of a novel porphyrin derivative in photodynamic therapy

作者：Li Jian Wei; Wu Zhong Ming; Magetic Davor; Zhang Li Jun; Chen Zhi Long^*

来源：Tumor Biology, 2015, 36(12): 9685-9692.

DOI：10.1007/s13277-015-3745-z

• TDPP
• Porphyrin
• Tumor
• Photosensitizer
• Photodynamic therapy

In this paper, the antitumor activity of a novel porphyrin-based photosensitizer 5,10,15,20-tetrakis[(5-diethylamino)pentyl] porphyrin (TDPP) was reported in vitro and in vivo. The photophysical and cellular properties of TDPP were investigated. The singlet oxygen generation quantum yield of TDPP was detected; it showed a high singlet oxygen quantum yield of 0.52. The intracellular distribution of photosensitizer was detected with laser scanning confocal microscopy. The efficiency of TDPP-photodynamic therapy (PDT) in vitro was analyzed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and in situ trypan blue exclusion test. Treated with a 630-nm laser, TDPP can kill cultured human esophageal cancer cell line (Eca-109) cells and reduce the growth of Eca-109 xenograft tumors significantly in BABL/c nude mice. And histopathological study was also used to confirm the antitumor effect. It has the perspective to be developed as a new antitumor drug in photodynamic therapy and deserves further investigation.

• 出版日期2015-12
• 单位东华大学