A Distinct Replication Fork Protection Pathway Connects Fanconi Anemia Tumor Suppressors to RAD51-BRCA1/2

作者：Schlacher, Katharina^*; Wu, Hong; Jasin, Maria

来源：Cancer Cell, 2012, 22(1): 106-116.

DOI：10.1016/j.ccr.2012.05.015

• CROSS-LINK REPAIR
• STRAND-BREAK REPAIR
• DNA-DAMAGE
• BLOOMS-SYNDROME
• CELLS
• CANCER
• PROTEIN
• GENOME
• FANCD2
• BRCA2

Genes mutated in patients with Fanconi anemia (FA) interact with the DNA repair genes BRCA1 and BRCA2/FANCD1 to suppress tumorigenesis, but the molecular functions ascribed to them cannot fully explain all of their cellular roles. Here, we show a repair-independent requirement for FA genes, including FANCD2, and BRCA1 in protecting stalled replication forks from degradation. Fork protection is surprisingly rescued in FANCD2-deficient cells by elevated RAD51 levels or stabilized RAD51 filaments. Moreover, FANCD2-mediated fork protection is epistatic with RAD51 functions, revealing an unanticipated fork protection pathway that connects FA genes to RAD51 and the BRCA1/2 breast cancer suppressors. Collective results imply a unified molecular mechanism for repair-independent functions of FA, RAD51, and BRCA1/2 proteins in preventing genomic instability and suppressing tumorigenesis.

• 出版日期2012-7-10