Decreased expression of Calpain-9 predicts unfavorable prognosis in patients with gastric cancer

作者：Peng, Peike; Wu, Weicheng; Zhao, Junjie; Song, Shushu; Wang, Xuefei; Jia, Dongwei; Shao, Miaomiao; Zhang, Mingming; Li, Lili; Wang, Lan; Duan, Fangfang; Zhao, Ran; Yang, Caiting; Wu, Hao; Zhang, Jie; Shen, Zhenbin^*; Ruan, Yuanyuan^*; Gu, Jianxin

来源：Scientific Reports, 2016, 6(1): 29604.

DOI：10.1038/srep29604

• PROSTATE
• BIOLOGY

Calpain-8 and calpain-9 belong to the family of calcium-dependent cysteine proteases, which are highly expressed in the stomach. However, the roles of calpain-8 and calpain-9 in gastric tumorigenesis remain little understood. Herein, we demonstrated that calpain-9 was generally decreased in gastric cancer cell lines and primary tumor tissues, while calpain-8 expression was not significantly altered. Calpain-9, but not calpain-8, induced cell cycle arrest in the G1 phase and cellular apoptosis in vitro, and it attenuated the growth of subcutaneous tumor xenografts in vivo. Low expression of calpain-9 was positively associated with male sex, late T stage, lymph node metastasis, and advanced TNM stage. Further analysis identified calpain-9 as an independent prognostic factor for poor prognosis, and combining calpain-9 with TNM stage generated a better predictive model for patient outcomes. In conclusion, calpain-9 is a tumor suppressor that can be regarded as a potential prognosis indicator for clinical outcomes in gastric cancer.

• 出版日期2016-7-12
• 单位复旦大学