A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers

作者：Zhang, Xiaohan; Zhuang, Huisheng^*

来源：Analytical Sciences, 2017, 33(12): 1441-1446.

DOI：10.2116/analsci.33.1441

• Carbon nanotubes
• immunoassay
• biotin-streptavidin-system
• polybrominated diphenyl ethers

The extensive use of polybrominated diphenyl ethers (PBDEs) has resulted in its increasingly widespread presence. Especially the lower halogenated PBDEs accumulate to a greater degree than the higher halogenated PBDEs in house dust, sewage sludge, pets, or even humans. In the present work, we developed an ultrasensitive biotin-streptavidin-enhanced carbon nanotube amplification strategy for the immunodetection of PBDEs, in which single-walled carbon nanotubes were used to immobilize numerous streptavidin. Meanwhile, we used biotin conjugated horseradish peroxidase (B-HRP) and biotin conjugated Goat anti-rabbit (B-IgG) to link the HRP and IgG to CNTs by using a biotin-streptavidin system. The sensitivity of the streptavidin-biotin-IgG-CNTs-HRP bioconjugate was compared with a commercial HRP-labelled IgG by using indirect competitive ELISA. The limit of this proposed ELISA detection (IC10) was 0.0059 ng/mL, showing a 20-time lower detection limit over the commercial one (IC10 = 0.1193 ng/mL). Finally, we applied the assay to the detection of PBDEs in dust samples. The results were consistent with those using GC-ECD, which confirmed that the proposed amplification strategy was accurate and receptive. This proposed biotin-streptavidin-enhanced carbon nanotube amplification strategy would be useful for ultrasensitive immunodetection in environmental studies.

• 出版日期2017-12
• 单位上海交通大学