Fluidization, resolidification, and reorientation of the endothelial cell in response to slow tidal stretches

作者：Krishnan Ramaswamy; Canovic Elizabeth Peruski; Iordan Andreea L; Rajendran Kavitha; Manomohan Greeshma; Pirentis Athanassios P; Smith Michael L; Butler James P; Fredberg Jeffrey J; Stamenovic Dimitrije^*

来源：American Journal of Physiology - Cell Physiology, 2012, 303(4): C368-C375.

DOI：10.1152/ajpcell.00074.2012

• contractile moment
• tractions
• cell shape
• cytoskeleton
• morphology

Krishnan R, Canovic EP, Iordan AL, Rajendran K, Manomohan G, Pirentis AP, Smith ML, Butler JP, Fredberg JJ, Stamenovic D. Fluidization, resolidification, and reorientation of the endothelial cell in response to slow tidal stretches. Am J Physiol Cell Physiol 303: C368-C375, 2012. First published June 13, 2012; doi:10.1152/ajpcell.00074.2012.-Mechanical stretch plays an important role in regulating shape and orientation of the vascular endothelial cell. This morphological response to stretch is basic to angiogenesis, neovascularization, and vascular homeostasis, but mechanism remains unclear. To elucidate mechanisms, we used cell mapping rheometry to measure traction forces in primary human umbilical vein endothelial cells subjected to periodic uniaxial stretches. Onset of periodic stretch of 10% strain amplitude caused a fluidization response typified by attenuation of traction forces almost to zero. As periodic stretch continued, the prompt fluidization response was followed by a slow resolidification response typified by recovery of the traction forces, but now aligned along the axis perpendicular to the imposed stretch. Reorientation of the cell body lagged reorientation of the traction forces, however. Together, these observations demonstrate that cellular reorientation in response to periodic stretch is preceded by traction attenuation by means of cytoskeletal fluidization and subsequent traction recovery transverse to the stretch direction by means of cytoskeletal resolidification.

• 出版日期2012-8