Aggregation of Whey Protein Hydrolysate Using Alcalase 2.4 L

作者：Liu, Chunhong; Liu, Wen; Feng, Zhibiao^*; Li, Dongmei

来源：PLos One, 2014, 9(10): e109439.

DOI：10.1371/journal.pone.0109439

• PLASTEIN REACTION
• INFRARED-SPECTROSCOPY
• SOY PROTEIN
• MECHANISM
• GLYCININ
• PAPAIN
• PH

Here, we describe peptide aggregation, which is also known as enzymatic protein resynthesis. Whey protein hydrolysate (WPH) is the starting material for assembling peptides. Analyses of the involved amino acids, intrinsic fluorescence, fluorescence phase diagram, secondary structure, turbidity, and surface hydrophobicity were performed to investigate the reaction process. The aggregation mechanism consists of two parts: 1) formation and 2) aggregation of the building blocks that form the ordered secondary beta-sheet structure. Constructing the building blocks requires at least one intermediate state, which is formed after 0.5 hours. Non-synergistic changes in the secondary and tertiary structures then allow the intermediate state to emerge.

• 出版日期2014-10-7
• 单位东北农业大学