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摘要
Seven water-soluble vitamins: thiamine hydrochloride (B-1), riboflavin hydrochloride (B-2), pyridoxine hydrochloride (B-6), calcium pantotenate (B-5), ascorbic acid (C), folic acid (B-9), and biotin (H, B-7) were separated using the micellar electrokinetic chromatographic (MEKC) method in a single run with the electrolyte solution consisting of 100 mM H3BO3, 5 mM Na2B4O7, and 30 mM sodium dodecylsulphate (SDS). The separation was achieved within 8 min. and the detection was performed at 200 nm. The calibration graphs plotted with six concentrations of each vitamin were linear with the determination coefficient r > 0.999. The method was applied for quantification of vitamins B-1, B-2, B-5, B-6, B-9, C, and H in novel feed for laboratory animals. The sample preparation involved solid-phase extraction of the vitamins, used prior to the MEKC analysis. The attained precision level was good with the recoveries between 98.4% and 105.6%. The simplicity of the procedure should make it highly useful for feed quality control in husbandry and the animal feed industry.
- 出版日期2012
