A novel DTPA cross-linking of hyaluronic acid metal complexation thereof

作者：Buffa Radovan^*; Betak Jiri; Kettou Sofiane; Hermannova Martina; Pospisilova Lucie; Velebny Vladimir

来源：Carbohydrate Research, 2011, 346(13): 1909-1915.

DOI：10.1016/j.carres.2011.04.015

• Hyaluronic acid
• Complex
• DTPA
• Metal
• Cross-linking

Macromolecular conjugates of a natural polysaccharide, hyaluronic acid, with diethylenetriaminepentaacetic acid (DTPA)-metal complexes were synthesized and characterized by FTIR, NMR, SEC-MALLS and ICP analysis. Several parameters of the cross-linking reaction as molecular weight of starting HA, temperature, equivalent of DTPA bis-anhydride, concentration of HA, presence of transacylation catalyst DMAP and reaction time were studied. The mechanism for the reaction was suggested and relationship between the molecular weight assigned by SEC-MALLS, reaction parameters and rheological properties of the final cross-linked products were investigated.