Dual growth factor-immobilized microspheres for tissue reinnervation: in vitro and preliminary in vivo studies

作者：Kim Tae Ho; Oh Se Heang; An Dan Bi; Lee Ji Youl; Lee Jin Ho^*

来源：Journal of Biomaterials Science, Polymer Edition, 2015, 26(5): 322-337.

DOI：10.1080/09205063.2015.1008882

• nerve growth factor (NGF)
• nerve regeneration
• neurogenic differentiation
• basic fibroblast growth factor (bFGF)
• microsphere

Growth factors (GFs) (basic fibroblast growth factor (bFGF) and/or nerve growth factor (NGF))-immobilized polycaprolactone (PCL)/Pluronic F127 microspheres were prepared using an isolated particulate-melting method and the sequential binding of heparin and GFs onto the microspheres. The GFs immobilized on the microspheres were released in a sustained manner over 28days, regardless of GF type. From the in vitro culture of muscle-derived stem cells, it was observed that the NGF-immobilized microspheres induced more neurogenic differentiation than the bFGF-immobilized microspheres, as evidenced by a quantitative real-time polymerase chain reaction using specific neurogenic markers (Nestin, GFAP, beta-tubulin, and MAP2) and Western blot (Nestin and beta-tubulin) analyses. The dual bFGF/NGF-immobilized microspheres showed better neurogenic differentiation than the microspheres immobilized with single bFGF or NGF. From the preliminary animal study, the dual bFGF/NGF-immobilized microsphere group also showed effective nerve regeneration, as evaluated by immunocytochemistry using a marker - beta-tubulin. The dual bFGF/NGF-immobilized PCL/Pluronic F127 microspheres may be a promising candidate for nerve regeneration in certain target tissues (i.e. muscles) leading to sufficient reinnervation.

• 出版日期2015-3-24