Assessment of copy-number variation in the NKG2C receptor gene in a single-tube and characterization of a reference cell panel, using standard polymerase chain reaction

作者：Moraru M; Canizares M; Muntasell A; de Pablo R; Lopez Botet M; Vilches C^*

来源：Tissue Antigens, 2012, 80(2): 184-187.

DOI：10.1111/j.1399-0039.2012.01911.x

• copy-number variation
• gene deletion
• genotyping
• human
• NK-cell receptors
• polymerase-chain reaction

Natural killer (NK) and T-lymphocytes monitor human leukocyte antigen (HLA)-E expression through CD94:NKG2 heterodimers. Structural polymorphism is not a hallmark for NK-complex genes on chromosome 12, except for complete NKG2C deletion in some humans. We present a method for fast, simple and accurate assessment of NKG2C copy-number variation presence or absence in the genome of an NKG2C gene, in homo- or heterozygosis, is detected by a single conventional polymerase chain reaction that yields amplicons of different lengths in each genotype. We have also determined the NKG2C genotypes of a reference cell panel comprising 13 NK- and tumour-cell lines and 39 Epstein-Barr virus transformed cells from the International Histocompatibility Workshop. Our results should facilitate research on the importance of NKG2C and its deletion for immunity.

• 出版日期2012-8