Determination of cellulose derived 5-hydroxymethyl-2-furfural content in lignocellulosic biomass hydrolysate by headspace gas chromatography

作者：Hu, Hui-Chao^*; Zhang, Yu; Zeng, Tong; Zhou, Weining; Chen, Lihui^*; Huang, Liulian; Ni, Yonghao

来源：Cellulose, 2018, 25(7): 3843-3851.

DOI：10.1007/s10570-018-1857-5

• 5-Hydroxymethyl-2-furfural
• Adsorption of furfural and 5-HMF
• Condensed lignin and humins
• Ethanol assisted extraction
• Headspace gas chromatography

This paper reports a robust method for rapid determination of cellulose derived 5-hydroxymethyl-2-furfural (5-HMF) in biomass hydrolysate suspension based on headspace gas chromatography (HS-GC). The headspace equilibration at 150 A degrees C for longer than 4 min with a pH range of 2.0-3.5 and a 50% (v/v) of ethanol content can obtain the full evaporation of 5-HMF, and eliminate the formation of additional 5-HMF generation from hexose. The linear GC response of 5-HMF was achieved by controlling the sample size within 20 mu L. The results showed that this method has a good precision (relative standard deviation < 6.97%) and accuracy (recoveries ranged from 94.0 to 104.8%). The limit of quantification of the method was 51 mg/L. It was also found that a considerable amount of furfural (13.8%) and 5-HMF (18.7%) was adsorbed on condensed lignin and humins in residual biomass, and the washing procedure with ethanol can completely extract the adsorbed furfural and 5-HMF. The present method is accurate, simple, and robust, it will be a powerful tool for determining the content of 5-HMF in the liquid samples from biorefinery related processes.

• 出版日期2018-7
• 单位福建农林大学