mRNA expression in osteoclasts: a chronobiological approach

作者：Hong, Zhang Qing; Tao, Liu Meng^*; Li, Liu

来源：Biological Rhythm Research, 2011, 42(4): 287-298.

DOI：10.1080/09291016.2010.503441

• osteoclast
• H+-ATPase
• carbonic anhydrase
• chronobiology
• Biological Rhythm Research

The objective of the present study was to investigate chronobiological rhythms of H+-ATPase and carbonic anhydrase II mRNA expression in rat osteoclasts. Rat osteoclasts were obtained through anatomical dissociation technique from bones. Experimental groups included 3 h, 6 h, 12 h, 24 h and 48 h cell cultures. mRNA expression of Atp6a1 and Atp6i of H+-ATPase and carbonic anhydrase II in osteoclasts were detected via fluorescent probe q-PCR. The data were analyzed using ANOVA with SPSS 12.0. mRNA expression of H+-ATPase and carbonic anhydrase II increased when the cell culture time was prolonged, and reached a peak value at 24 h; however later cultures resulted in a significant decrease in mRNA expression. Furthermore, mRNA expression of H+-ATPase and carbonic anhydrase II in osteoclasts varied with time of culture.

• 出版日期2011
• 单位浙江大学