Differential requirement of ribosomal protein S6 by plant RNA viruses with different translation initiation strategies

作者：Yang Chunling; Zhang Chunquan; Dittman Jaime D; Whitham Steven A^*

来源：Virology, 2009, 390(2): 163-173.

DOI：10.1016/j.virol.2009.05.018

• Ribosomal protein
• Plant virus
• RPS6
• RPL19
• Potyvirus
• Tobamovirus
• Tombusvirus

Potyvirus infection has been reported to cause an increase in the mRNA transcripts of many plant ribosomal proteins (r-proteins). In this Study, increased expression of r-protein mRNA transcripts was determined to occur in Nicotiana benthamiana during infection by potyviruses as well as a tobamovirus demonstrating that this response is not unique to potyviruses. Five r-protein genes, RPS6, RPL19, RPL13, RPL7, and RPS2. were silenced in N. benthamiana to test their roles in viral infection. The accumulation of both Turnip mosaic virus (TuMV), a potyvirus, and Tobacco mosaic virus (TMV), a tobamovirus, was dependent on RPL19, RPL13, RPL7, and RPS2. However, TMV was able to accumulate in RPS6-silenced plants while accumulation of TuMV and Tomato bushy stunt virus (TBSV) was abolished. These results demonstrate that cap-independent TuMV and TBSV require RPS6 for their accumulation, whereas accumulation of TMV is independent of RPS6.

• 出版日期2009-8-1