Annexin 1 protects against apoptosis induced by serum deprivation in transformed rat retinal ganglion cells

作者：Shao Zhengbo; Shen Jing; Yang Yuanhang; Wu Donglai; Zhou Xuemei; Yuan Huiping^*

来源：Molecular Biology Reports, 2012, 39(5): 5543-5551.

DOI：10.1007/s11033-011-1358-1

• Annexin 1
• Retinal ganglion cells
• Apoptosis
• Ac2-26
• Neuroprotection

To investigate whether Annexin 1 can protect a retinal ganglion cells line (RGC-5) from apoptosis as induced by serum deprivation. Annexin 1 location in RGC-5 cells was determined using an indirect immunofluorescent assay. Expression of Annexin 1 in RGC-5 cultures deprived of serum for 0, 2 days was semi-quantified by western blot and RT-PCR. Effects of varying concentrations of the Annexin 1 peptide fragment, Ac2-26, on the survival of the RGC-5 cells was determined, and apoptotic cells were quantified by flow cytometry. Immunoblot and RT-PCR analysis was preformed to identify caspase 3, bax and bcl-2 in RGC extracts. Annexin 1 was localized in the cytoplasm of RGC-5 cells and the expression of Annexin 1, caspase 3 and bax was upregulated in serum-deprived RGC-5 cells. Ac2-26 attenuated the apoptosis resulting from serum deprivation of RGC-5 in a concentration-dependent manner, decreased caspase 3 and bax levels and produced an increase of bcl-2 in cell lysates. Annexin 1, in specific the peptide fragment Ac2-26, may play an important role in decreasing apoptosis in serum-deprived RGC-5 cells.

• 出版日期2012-5
• 单位哈尔滨医科大学