An automated microfluidic sample preparation system for laser scanning cytometry

作者：Wu Eric; Menon Vidya; Geddie William; Sun Yu^*

来源：Biomedical Microdevices, 2011, 13(2): 393-401.

DOI：10.1007/s10544-010-9508-0

• Laser scanning cytometry
• Microfabrication
• Microfluidic dispenser
• Microfluidic connection
• Microfluidic module
• Capillary burst valve
• Passive valve
• Porous membrane
• Sample preparation
• Automation

Laser scanning cytometry (LSC) is emerging as a clinical tool. In one application a "Clatch" slide, named after the inventor, is used in conjunction with LSC for cell surface marker immunophenotyping of patient samples. The slide requires time consuming and laborious pipetting steps, making a test tedious and prone to handling errors. The Clatch slide also uses a significant number of cells, limiting the number of analyses on paucicellular samples. This paper presents an automated microfluidic system consisting of a control circuit, a microfluidic system, and an aluminum frame, capable of performing immunophenotyping procedures. This prototype system reduces 36 pipetting steps to 1, reduces the amount of cell sample from 180 mu L to 56 mu L, and shortens the time used by technicians.

• 出版日期2011-4