A novel reduced graphene oxide/molybdenum disulfide/polyaniline nanocomposite-based electrochemical aptasensor for detection of aflatoxin B-1

作者：Geleta, Girma Selale; Zhao, Zhen; Wang, Zhenxin^*

来源：Analyst, 2018, 143(7): 1644-1649.

DOI：10.1039/c7an02050c

• LAYERED MOS2-GRAPHENE COMPOSITES
• FOOD TOXIN DETECTION
• ULTRASENSITIVE DETECTION
• SIGNAL AMPLIFICATION
• SENSITIVE DETECTION
• AU NANOPARTICLES
• BIOSENSOR
• POLYANILINE
• PERFORMANCE
• PLATFORM

In this study, we developed a novel reduced graphene oxide/molybdenum disulfide/polyaniline@gold nanoparticles-based electrochemical aptasensor (termed as RGO/MoS2/PANI@AuNPs/Apt) for detection of aflatoxin B-1 (AFB(1)). The RGO/MoS2/PANI nanocomposites were synthesized and characterized by multiple techniques, including scanning electron microscopy (SEM), transmission electron microscopy (TEM), infrared spectroscopy (FTIR), UV-visible spectroscopy, and X-ray photoelectron spectroscopy (XPS). A glassy carbon electrode (GCE) was then modified by the RGO/MoS2/PANI nanocomposites, coated with a chitosan (Cs) film, and followed by AuNPs attachment for immobilizing the AFB(1) aptamers. In the presence of AFB(1), the AFB(1) binding-induced conformation change of the immobilized aptamer on the electrode surface results in the reduction of the electron transfer from a [Fe(CN)(6)](3-/4-) redox couple in the solution to the GCE surface. Therefore, the aptamer-AFB(1) binding event can be easily monitored by the peak current change of the RGO/MoS2/PANI@AuNPs/Apt through differential pulse voltammetry (DPV) measurement. Under the optimized conditions, the as-developed RGO/MoS2/PANI@AuNPs/Apt exhibits a wide linear range from 0.01 fg mL(-1) to 1.0 fg mL(-1) and a remarkably low detection limit (3 sigma) of 0.002 fg mL(-1). The aptasensor also has good reproducibility as well as shows high selectivity against other fungal toxins, such as OTA and FB1. Moreover, the practicability of the RGO/MoS2/PANI@AuNPs/Apt was demonstrated by the analysis of AFB(1) in the spiked wine samples.

• 出版日期2018-4-7
• 单位电分析化学国家重点实验室; 中国科学院; 中国科学院长春应用化学研究所; 中国科学院大学