Main pathways of action of Brazilian red propolis on the modulation of neutrophils migration in the inflammatory process

作者：Bueno Silva Bruno^*; Franchin Marcelo; Alves Claudiney de Freitas; Denny Carina; Colon David Fernando; Cunha Thiago Mattar; Alencar Severino Matias; Napimoga Marcelo Henrique; Rosalen Pedro Luiz

来源：Phytomedicine, 2016, 23(13): 1583-1590.

DOI：10.1016/j.phymed.2016.09.009

• Brazilian red propolis
• Neutrophils
• Cytokines
• Inflammation

Background: Brazilian propolis is popularly used as treatment for different diseases including the ones with inflammatory origin. Brazilian red propolis chemical profile and its anti-inflammatory properties were recently described however, its mechanism of action has not been investigated yet.
Aim: Elucidate Brazilian red propolis major pathways of action on the modulation of neutrophil migration during the inflammatory process.
Methods: The ethanolic extract of propolis (EEP) activity was investigated for neutrophil migration into the peritoneal cavity, intravital microscopy (rolling and adhesion of leukocytes), quantification of cytokines TNF-alpha, IL-1 beta and chemokines CXCL1/KC, CXCL2/MIP-2, neutrophil chemotaxis induced by CXCL2/MIP-2, calcium influx and CXCR2 expression on neutrophils.
Results: EEP at 10 mg/kg prevented neutrophil migration into peritoneal cavity (p < 0.05), reduced leukocyte rolling and adhesion on the mesenteric microcirculation (p < 0.05) and inhibited the release TNF-alpha, IL-1 beta, CXCL1/KC and CXCL2/MIP-2 (p < 0.05). EEP at 0.01, 0.1 and 1 mu g/ml reduced the CXCL2/MIP-2-induced neutrophils chemotaxis (p < 0.05) without affect cell viability (p > 0.05). EEP at 1 mu g/ml decreased the calcium influx induced by CXCL2/MIP-2 (p < 0.05). On the other hand, none of EEP concentrations tested altered CXCR2 expression by neutrophils (p > 0.05).
Conclusion: Brazilian red propolis appears as a promising anti-inflammatory natural product which mechanism seems to be by reducing leukocyte rolling and adhesion; TNF-alpha, IL-1 beta, CXCL1/KC and CXCL2/MIP-2 release; CXCL2/MIP-2-induced chemotaxis and calcium influx.

• 出版日期2016-12-1