Assessing next-generation sequencing and 4 bioinformatics tools for detection of Enterovirus D68 and other respiratory viruses in clinical samples

作者：Huang Weihua^*; Wang Guiqing; Lin Henry; Zhuge Jian; Nolan Sheila M; Vail Eric; Dimitrova Nevenka; Fallon John T

来源：Diagnostic Microbiology and Infectious Disease, 2016, 85(1): 26-29.

DOI：10.1016/j.diagmicrobio.2016.01.013

• Enterovirus D68
• Next-generation sequencing
• Bioinformatics
• Diagnostic sensitivity
• Diagnostic specificity

We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feasible and useful for clinical diagnosis of viral infections.

• 出版日期2016-5